Commercial Reagent Kit of RamDA-seq is available.

We developed random displacement amplification sequencing (RamDA-seq), the first full-length total RNA-sequencing method for single cells. Compared with other methods, RamDA-seq shows high sensitivity to non-poly(A) RNA and near-complete full-length transcript coverage.

Protocol

• Detail Protocol of RamDA-seq / C1 RamDA-seq
  • Lastest version 1.2, 2020/03/19
  • Version 1.1, 2018/09/24
• List of not-so-random primer (Human/Mouse, xlsx)
• C1 RamDA-seq (Fluidigm C1 ScriptHub, 2018/09/17)

Data

• SRA files and expression matrix of RamDA-seq (NCBI GEO)
• FASTQ files (ENA)

Data Analysis Pipeline

• RamDAQ: Quality control pipeline for RamDA-seq experiments

Citation

Hayashi T*, Ozaki H*, Sasagawa Y, Umeda M, Danno H, and Nikaido I.
Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs.
Nature Communications. 2018.

Media

• Researchers successfully sequence total RNA of single cells. RIKEN Press Release. 2018/03/06.

If you need a high-sensitive and throughput single-cell RNA-seq, please see Quartz-Seq2 page.

Contact

support-bit (at) riken (dot) jp