Quartz-Seq

Quartz-Seq2’s Protocol is here.

1. Quartz-Seq: Single-Cell RNA-seq (2-13 pg Total RNA)

Update: 2014/04/20

Reference and Data

2. Quartz-Seq: RNA-Seq for low amount total RNA (100 pg – 1 ng, 10 – 1000 cells)

Update: 2015/11/09

3. DNA Sequencing Library Preparation for illumina HiSeq

Update: 2015/02/05

Citation

Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity
Yohei Sasagawa, Itoshi Nikaido, Tetsutaro Hayashi, Hiroki Danno, Kenichiro D Uno, Takeshi Imai and Hiroki R Ueda
Genome Biology201314:3097

Movies (Japanese language)

  1. 試薬の管理編_Quartz-Seq Next96
  2. 0.4uL Lysis bufferの分注手技_Quartz-Seq Next96
  3. PCR plateのフタを利用した溶液の分注方式の説明_Quartz-Seq Next96
  4. 細胞を採取したLysis bufferを-80℃で保存するまでの工程_Quartz-Seq Next96
  5. Priming step_Quartz-seq Next96