Protocol

• Detail Protocols of RamDA-seq / C1-RamDA-seq (Version 1.1, 2018/09/24)
• List of not-so-random primer (Human/Mouse, xlsx)
• C1 RamDA-seq (Fluidigm C1 ScriptHub, 2018/09/17)

Data

• SRA files and expression matrix of RamDA-seq (NCBI GEO)
• FASTQ files (ENA)

FAQ

Q1. Can I change from X reagent to Y reagent?

A1. No. It’s no longer RamDA-seq. We cannot support your original scRNA-seq method 🙂

Citation

Hayashi T*, Ozaki H*, Sasagawa Y, Umeda M, Danno H, and Nikaido I.
Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs.
Nature Communications. 2018.

Media

• Researchers successfully sequence total RNA of single cells. RIKEN Press Release. 2018/03/06.

Contact

support-bit (at) riken (dot) jp