We developed random displacement amplification sequencing (RamDA-seq), the first full-length total RNA-sequencing method for single cells. Compared with other methods, RamDA-seq shows high sensitivity to non-poly(A) RNA and near-complete full-length transcript coverage.
- Detail Protocol of RamDA-seq / C1 RamDA-seq
- List of not-so-random primer (Human/Mouse, xlsx)
- C1 RamDA-seq (Fluidigm C1 ScriptHub, 2018/09/17)
Data Analysis Pipeline
Q1. Can I change from X reagent to Y reagent?
A1. No. It’s no longer RamDA-seq. We cannot support your original scRNA-seq method 🙂
- Researchers successfully sequence total RNA of single cells. RIKEN Press Release. 2018/03/06.
support-bit (at) riken (dot) jp