Quartz-Seq2’s Protocol is here.

1. Quartz-Seq: Single-Cell RNA-seq (2-13 pg Total RNA)

Update: 2014/04/20

• Whole-transcript amplification for single-cell Quartz-Seq (PDF)
• DNA Library preparation method for Single-Cell Quartz-Seq: LIMprep for Quartz-Seq (PDF)

Reference and Data

• A magnetic stand for ultra-low sample (YouTube)
• Quartz-Seq: a highly reproducible and sensitive single-cell RNA-Seq reveals non-genetic gene expression heterogeneity (Original Paper)
• All FPKM Matrix Data (tab separated text file, zip archive)
• Quartz-Seq.org: A database for single-cell Quartz-Seq with Embryonic stem cell

2. Quartz-Seq: RNA-Seq for low amount total RNA (100 pg – 1 ng, 10 – 1000 cells)

Update: 2015/11/09

• Whole-transcript amplification for cell-population Quartz-Seq (Cell Direct Version)
• Whole-transcript amplification for cell-population Quartz-Seq (Purified RNA Version)

3. DNA Sequencing Library Preparation for illumina HiSeq

Update: 2015/02/05

• LIMPrep2’s Protocol (recommended)
• LIMPrep’s Protocol (obsoleted)
  • Blog entry: LIMprep: a highly-sensitive multiplex library preparation method for low amount DNA
  • LIMprep Technical Note (PDF)

Movies (Japanese language)

1. 試薬の管理編_Quartz-Seq Next96
2. 0.4uL Lysis bufferの分注手技_Quartz-Seq Next96
3. PCR plateのフタを利用した溶液の分注方式の説明_Quartz-Seq Next96
4. 細胞を採取したLysis bufferを-80℃で保存するまでの工程_Quartz-Seq Next96
5. Priming step_Quartz-seq Next96